Authentication of Rusa timorensis DNA Using Sequencing Assay with Modified Extraction Method

A technique to isolate DNA from deer meat was established authenticate the species and successfully extracted meat. The method based on Epicentre MasterPureTM Complete RNA Purification Kit extraction kit with slight modification by increasing volume of Proteinase K RNase 5 µL 20 each. recovery then analysed Polymerase Chain Reaction using cytochrome b gene oligonucleotide primers for family, which targeted mitochondrial DNA. resulted in clean no smearing after performing Reaction. Furthermore, amplified product extended sequence assay above 95% maximum identification. result showed that offered a high yield recovery, provided more than 100 ng per μL useful validating animal-based sources, especially products.